Virus Binding and Internalization Assay for Adeno-associated Virus
نویسندگان
چکیده
منابع مشابه
Adeno-associated virus biology.
Adeno-associated virus (AAV) was first discovered as a contaminant of adenovirus stocks in the 1960s. The development of recombinant AAV vectors (rAAV) was facilitated by early studies that generated infectious molecular clones, determined the sequence of the genome, and defined the genetic elements of the virus. The refinement of methods and protocols for the production and application of rAAV...
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We have investigated the infectious entry pathway of adeno-associated virus (AAV) and recombinant AAV vectors by assessing AAV-mediated gene transfer and by covalently conjugating fluorophores to AAV and monitoring entry by fluorescence microscopy. We examined AAV entry in HeLa cells and in HeLa cell lines which inducibly expressed a dominant interfering mutant of dynamin. The data demonstrate ...
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Gene therapy for cancer offers novel treatment paradigms that will eventually lead to the destruction of tumor cells in patients with solid and hematopoietic malignancies. Major cancer gene therapy approaches that directly target tumor cells include chemosensitization, cytokine gene transfer, inactivation of proto-oncogene expression, replacement of defective tumor suppressor genes, and transdu...
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We constructed insertion and deletion mutants with mutations within the adeno-associated virus (AAV) sequences of the infectious recombinant plasmid pSM620. Studies of these mutants revealed at least three AAV phenotypes. Mutants with mutations between 11 and 42 map units were partially or completely defective for rescue and replication of the AAV sequences from the recombinant plasmids (rep mu...
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In this application note, we describe a novel method to rapidly measure the size and concentration of Adeno-Associated Virus (AAV) utilizing the Nanoparticle Tracking Analysis (NTA) technique and a gold labeling method. By labeling AAV capsids with small gold nanoparticles, we were able to increase the amount of light scattered by each capsid, enabling rapid NTA characterization to determine th...
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ژورنال
عنوان ژورنال: BIO-PROTOCOL
سال: 2017
ISSN: 2331-8325
DOI: 10.21769/bioprotoc.2110